1. Prepare monobasic sodium phosphate buffer by dissolving 4.22 g of sodium dihydrogen orthophosphate hydrate (NaH2PO4 · H2O) in 250 mL of ddH2O. Adjust the pH to ∼7.4.
2. In a hood, immediately before use, prepare 3% (v/v) formaldehyde by adding 1.5 mL of 37% (w/v) formaldehyde solution to 48.5 mL of monobasic sodium phosphate buffer.
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